https://nova.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:54316 30 µM TMAO in hearts performing minimal isovolumic work, although this response was reduced by >65%. In contrast, exposure to 10 µM or 100 μM TMAO increased mitochondrial complex I, II and maximal respiratory fluxes while appearing to reduce outer membrane integrity. Expression of phosphorylated AMPKα and total GSK-3β declined. Thus, acute exposure of mouse hearts to TMAO levels reported in advanced CVD significantly inhibits cardiac contractility and induces modest coronary constriction while paradoxically overactivating mitochondrial respiration.]]> Wed 28 Feb 2024 15:01:29 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:6544 Wed 11 Apr 2018 16:31:09 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:6546 Wed 11 Apr 2018 16:15:33 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:6551 Wed 11 Apr 2018 15:20:31 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:14146 Wed 11 Apr 2018 15:12:12 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:15241 + T cells. We found that a limited number of microRNAs exhibited marked up- or downregulation following early-life infection and sensitisation, for many of which the levels of expression were further changed following chronic challenge with the sensitizing antigen. Targets of these microRNAs included genes involved in immune or inflammatory responses (e.g. Gata3, Kitl) and in tissue remodelling (e.g. Igf1, Tgfbr1), as well as genes for various transcription factors and signalling proteins. In pulmonary CD4⁺ T cells, there was significant demethylation at promoter sites for interleukin-4 and interferon-γ, the latter increasing following chronic challenge. We conclude that, in this model, progression to an asthmatic phenotype is linked to epigenetic regulation of genes associated with inflammation and structural remodelling, and with T-cell commitment to a Th2 immunological response. Epigenetic changes associated with this pattern of gene activation might play a role in the development of childhood asthma.]]> Wed 11 Apr 2018 14:44:33 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:6552 Wed 11 Apr 2018 13:43:05 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:1350 Wed 11 Apr 2018 12:50:26 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:6543 Wed 11 Apr 2018 12:03:05 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:24259 Wed 11 Apr 2018 11:39:29 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:6548 Wed 11 Apr 2018 10:37:33 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:6550 Wed 11 Apr 2018 10:31:25 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:35130 98%) compared with supervised ANNs. AlexNet demonstrated the highest accuracy (99.55%) with negligible false classifications. The results indicate that sEMG quality evaluation can be automated via an ANN without compromising human-like classification accuracy. This classifier will be publicly available and will be a valuable tool for researchers and clinicians using electromyography.]]> Thu 02 Apr 2020 15:47:43 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:8198 Sat 24 Mar 2018 08:36:19 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:11246 Cdh1) activity, which ubiquitylates and so targets cyclin B1 for degradation. Thus, APC^Cdh1 activity prevents precocious meiotic entry by promoting cyclin B1 degradation. However, it remains unresolved how cyclin B1 levels are suppressed sufficiently to maintain arrest but not so low that they make oocytes hormonally insensitive. Here, we examined spatial control of this process by determining the intracellular location of the proteins involved and using nuclear-targeted cyclin B1. We found that raising nuclear cyclin B1 concentrations, an event normally observed in the minutes before nuclear envelope breakdown, was a very effective method of inducing the G2/M transition. Oocytes expressed only the α-isoform of Cdh1, which was predominantly nuclear, as were Cdc27 and Psmd11, core components of the APC and the 26S proteasome, respectively. Furthermore, APC^Cdh1 activity appeared higher in the nucleus, as nuclear-targeted cyclin B1 was degraded at twice the rate of wild-type cyclin B1. We propose a simple spatial model of G2 arrest in which nuclear APC^Cdh1-proteasomal activity guards against any cyclin B1 accumulation mediated by nuclear import.]]> Sat 24 Mar 2018 08:10:55 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:29533 2+-binding tyrosine-phosphorylation-regulated protein (CABYR) has been implicated in sperm physiological function in several in vitro studies. It has also been implicated as a potential cause of and diagnostic tool in asthenozoospermic human males. CABYR is known to be localized to the fibrous sheath, an accessory structure in the flagellar principal piece. Utilizing the CRISPR–Cas9 technology, we have knocked out this gene in mice to understand its role in male fertility. Cabyr-knockout male mice showed severe subfertility with a defect in sperm motility as well as a significant disorganization in the fibrous sheath. Further, abnormal configuration of doublet microtubules was observed in the Cabyr-knockout spermatozoa, suggesting that the fibrous sheath is important for the correct organization of the axoneme. Our results show that it is the role of CABYR in the formation of the fibrous sheath that is essential for male fertility.]]> Sat 24 Mar 2018 07:32:26 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:42509 Acridotheres tristis) and measured eye patch coloration as a function of the food combinations individuals selected. Mynas had access to either water or carotenoid-supplemented water and could either eat a standard captive diet or choose freely between three nutritionally defined pellets (protein, lipid or carbohydrate). Mynas supplemented with both carotenoids and macronutrient pellets had higher color scores than control birds. Male coloration tended to respond more to nutritional manipulation than females, with color scores improving in macronutrient-and carotenoid-supplemented individuals compared with controls. All mynas consuming carotenoids had higher levels of plasma carotenoids, but only males showed a significant increase by the end of the experiment. Dietary carotenoids and macronutrient intake consumed in combination tended to increase plasma carotenoid concentrations the most. These results demonstrate for the first time that consuming specific combinations of macronutrients along with carotenoids contributes to optimizing a colorful signal, and point to sex-specific nutritional strategies. Our findings improve our knowledge of how diet choices affect signal expression and, by extension, how nutritionally impoverished diets, such as those consumed by birds in cities, might affect sexual selection processes and, ultimately, population dynamics.]]> Mon 28 Nov 2022 15:02:47 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:48524 Acropora muricata High light (HL)- and low light (LL)-acclimated corals were collected from the reef and reciprocally exposed to high and low light ex situ Rapid light curves using pulse-amplitude modulation (PAM) fluorometry revealed photophysiological acclimation of LL corals to HL and HL corals to LL within 21 days. A subset of colonies sampled at 7 and 21 days for untargeted LC-MS and GC-MS metabolomic profiling revealed metabolic reorganization before acclimation was detected using PAM fluorometry. Metabolomic shifts were more pronounced for LL to HL corals than for their HL to LL counterparts. Compounds driving metabolomic separation between HL-exposed and LL control colonies included amino acids, organic acids, fatty acids and sterols. Reduced glycerol and campesterol suggest decreased translocation of photosynthetic products from symbiont to host in LL to HL corals, with concurrent increases in fatty acid abundance indicating reliance on stored lipids for energy. We discuss how these data provide novel insight into environmental regulation of metabolism and implications for management strategies that drive rapid changes in light availability.]]> Mon 20 Mar 2023 17:56:36 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:38687 Fri 17 Dec 2021 14:01:20 AEDT ]]>